Coding

Part:BBa_K4606003

Designed by: Baochao Li   Group: iGEM23_Nanjing-NFLS   (2023-09-14)


SMAD4(wild type)

Description

SMAD4 is important for cartilage homeostasis. The inhibition of SMAD4 can induce chondrocyte apoptosis and aggravate osteoarthritis progression. Based on the online repository of miRNA targets, SMAD4 might be a target of miR-3074-5p in osteoarthritis.

We constructed a portion of the gene encoding SMAD4. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment. This fusion expression does nearly no harm to the normal physiological state of firely luciferase.

Figure2 (Part3). Acquisition of SMAD4-WT gene segments by PCR.
1#: DNA marker. 2#: Segments of target gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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